The triphosphoinositide phosphomonoesterase of brain tissue
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The triphosphoinositide phosphomonoesterase of brain tissue.
Preparation of triphosphoinositide phosphomonoesterase from brain extract8. To 40 ml. of the extract of acetone-dried brain powder (Thompson & Dawson, 1964a) was added 20 ml. of 0 132M-tris-HCl buffer, pH 7-2, and the mixture was cooled to 00. Solid (NH4)2SO4 (10-64 g.) was slowly added with stirring, and the mixture was left for 30 min. at 00. After centrifuging at 0° for 10 min. at 10000gay. ...
متن کاملThe triphosphoinositide phosphodiesterase of brain tissue.
Preparation of triphosphoinositide phosphodiesterase from brain extracts. The dialysed aqueous extract ofacetone-dried brain powder (Thompson & Dawson, 1964) was held in a water bath at 500 for 30 min. After cooling in an ice bath 0-25 vol. of 0(132 M-tris-HCl buffer, pH 7-2, was added. Saturated (NH4)2SO4 solution (neutralized to pH 7) at 40 was slowly added with stirring to give 30% saturatio...
متن کاملThe Subcellular Distribution of Triphosphoinositide
1. Some properties of the triphosphoinositide phosphomonoesterase from the homogenates of guinea-pig brain were studied. The enzyme has an optimum pH range 6.7-7.3, is stimulated with KCl at a concentration of 0.1M, and under these conditions has K. 1.43 x 10-4M. 2. A factor from the 'pH5 supernatant' of guinea-pig brain stimulates the enzyme activity over and above the stimulation produced by ...
متن کاملThe diphosphoinositide kinase of rat brain.
1. The supernatant fraction of adult rat brain contains a diphosphoinositide kinase. 2. Formation of triphosphoinositide by the enzyme in the presence of ATP and Mg(2+) ions was shown with labelled ATP or labelled diphosphoinositide. 3. The kinase was also activated by Ca(2+), Mn(2+) and Co(2+) ions, but to a smaller extent than by Mg(2+) ions. 4. In the presence of optimum Mg(2+) ion concentra...
متن کاملThe isolation of a new lipid, triphosphoinositide, and monophosphoinositide from ox brain.
Massey, V. (1953). Biochem. J. 58, 72. Moelwyn-Hughes, E. A. (1947). Kinetics of Reactions in Solution, 2nd ed., p. 5. Oxford University Press. Mommaerts, W. F. H. M. (1950). Muscular Contraction, chap. 3. New York: Interscience Publishers Inc. Mommaerts, W. F. H. M. & Green, I. (1954). J. biol. Chem. 208, 833. Morales, M. F. & Botts, J. (1956). In Currents in Biochemical Research, p. 609. Ed. ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1964
ISSN: 0006-2936
DOI: 10.1042/bj0910244